ELISA plate for 96 measurements
Scientific Background
ANXA1, or Lipocortin I, is a 37 kDa protein from the annexin family with strong anti-inflammatory and pro-resolving activities. Like most members it binds negatively charged phospholipids in Ca2+-dependent manner. In addition, it binds the FPR2/ALX receptor and elicits anti-inflammatory responses upon FPR2/ALX ligation. ANXA1 is regarded as an important mediator of resolution of acute inflammation. Blood plasma ANXA1 originates mostly from activated and dying cells of the innate and adaptive immune system, including neutrophils, monocytes, macrophages, and dendritic cells. Plasma ANXA1 reflects ongoing inflammation and an activated immune system, especially in cancer. Plasma ANXA1 can be proteolytically cleaved into an N-terminal domain of around 3 kDa and a C-terminal domain of circa 34 kDa. Both the full-length ANXA1 and the 34 kDa C-terminal domain can be measured in citrated plasma using the sandwich ELISA. Plasma ANXA1 is a useful biomarker for immune-inflammatory states associated with infectious diseases, malignancy and autoimmunity.
Test Principle
The 5-ELISA Anti-Annexin A1, IgG Autoantibody kit is a sandwich ELISA with recombinant Annexin A1 antigen coated on the plate for capturing Anti-Annexin A1 auto-antibodies in the test sample. Captured IgG autoantibodies are tagged with a peroxidase-labelled polyclonal antibody, which binds on them in a dose-dependent manner. Tetramethylbenzidine (TMB) with hydrogen peroxide (H2O2) is used for the color development. Results are expressed with the measured A450 and compared to the normal range (Mean +/- 2 SDs)
The test kit is for research use only (RUO) and not for diagnostic applications and not for use in humans.
Characteristics
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