5-Elisa ACE 2 Antigen

Art. No.: 5D-55901

ELISA plate for 96 measurements

Product Description
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Scientific Background

ACE2, is a cell surface receptor, present on many cell types. It lowers blood pressure by converting angiotensin II (a vasoconstrictor) to angiotensin 1-7, a vasodilator. It is a major player of the Renin-Angiotensin-Aldosterone System (RAAS). ACE2 is also present in the soluble form in blood circulation, as the result of its extracellular domain cleavage by ADAM 17, releasing it from cell surface. The soluble form is essential for cleaving angiotensin II and lowering blood pressure. Increased concentrations of soluble ACE2 can be associated to various cardiovascular diseases.

Test Principle

This sandwich ELISA is designed with polyclonal antibodies coated onto the plate for capturing soluble forms of Angiotensin Converting Enzyme 2 (ACE2) in the tested sample. Following a washing step, captured ACE2 is tagged with a peroxidase-labelled polyclonal antibody, which binds onto its free epitopes in a dose-dependent manner. Tetramethylbenzidine (TMB) with hydrogen peroxide (H2O2) is used for the color development.

Characteristics

Measuring range 0 - 100 ng/mL
Color development is measured at 450 nm
Measured value is directly proportional to the amount of ACE2 present in the sample
Results are expressed in ng/ml, by reference to a calibrated internal reference preparation of human recombinant ACE2.

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