5-Elisa Annexin A1 (ANXA1 or Lipocortin)

Art. No.: 5D-55902

ELISA plate for 96 measurements

Product Description
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Scientific Background

ANXA1, or Lipocortin I, is a 37 kDa protein from the annexin family with strong anti-inflammatory and pro-resolving activities. Like most members it binds negatively charged phospholipids in Ca2+-dependent manner. In addition, it binds the FPR2/ALX receptor and elicits anti-inflammatory responses upon FPR2/ALX ligation. ANXA1 is regarded as an important mediator of resolution of acute inflammation. Blood plasma ANXA1 originates mostly from activated and dying cells of the innate and adaptive immune system, including neutrophils, monocytes, macrophages, and dendritic cells. Plasma ANXA1 reflects ongoing inflammation and an activated immune system, especially in cancer. Plasma ANXA1 can be proteolytically cleaved into an N-terminal domain of around 3 kDa and a C-terminal domain of circa 34 kDa. Both the full-length ANXA1 and the 34 kDa C-terminal domain can be measured in citrated plasma using the sandwich ELISA. Plasma ANXA1 is a useful biomarker for immune-inflammatory states associated with infectious diseases, malignancy and autoimmunity.

Test Principle

The 5-ELISA Annexin A1 Antigen kit is a two-site enzyme immunoassay for measuring Annexin A1 antigen in plasma or in any biological fluid.This sandwich ELISA is designed with polyclonal antibodies coated onto the plate for capturing ANNEXIN A1 (ANXA1) in the tested sample. Captured ANXA1 is tagged with a peroxidase-labelled polyclonal antibody, which binds onto the free epitopes in a dose-dependent manner. Tetramethylbenzidine (TMB) with hydrogen peroxide (H2O2) is used for the color development.

Characteristics

Measuring range 0 - 50 ng/mL
Color development is measured at 450 nm
Measured value is directly proportional to the amount of ANXA1 present in the sample
Results are expressed in ng/ml, by reference to a calibrated internal reference preparation of human recombinant ANXA1.

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