XenoScreen XL YAS - Yeast Androgen Screen - Test Kit for the Detection of Endocrine Disruptive Chemicals

Art. No.: N32-234Y

4 samples in 1 receptor (estrogen activity) if tested in duplicates, 8 dilutions, controls. 32 samples in Estrogen receptor activity, duplicates, if tested in 1 dilution only. YES and YAS strains (up to 20 passages) need to be added separately. Additional YES YAS Culture Maintenance Set is available.

Product Description
Documents (3)

XenoScreen XL YAS is a ready to use androgen receptor transactivation test kit for the identification of chemicals, agrochemicals, cosmetics (EDC's) or water samples with endocrine disruptive activity, i.e estrogenic activity. In the receptor transactivation test a chemical is required to bind to the receptor and to activate the receptor. The test kit is used for measurement of agonistic activity only.

Many endocrine disrupting chemicals adversely impact the androgen signaling by interacting with androgen receptors. The receptors show ligand selectivity and target gene regulation, they modulate metabolic enzymes that are critical for normal androgen synthesis and metabolism. Typical EDC's are e.g. phytoestrogens, bisphenolics, and organochlorine pesticides. Many endocrine discrupting chemicals act through multiple mechanisms and therefore biological effects of EDCs need to be carefully interpreted.

In the receptor transcriptional activation assay, YAS (Yeast Androgen Screen) cells are exposed to 1 or 8 concentrations of a test sample, as well as a positive and a negative control, for 18 hours. After exposure, the receptor mediated β-galactosidase expression is assessed via a chromogenic substrate. Each dose is tested in duplicate to allow for statistical analysis of the data. Alternatively 32 compounds can be tested if only 1 concentration is applied.

A dose-dependent and significant increase or decrease in the color development relative to the corresponding negative control upon exposure indicates that the sample acts as an endocrine disruptor. Comparison with standardized positive controls (tested in multiple doses) allows for a quantification of the endocrine disrupting properties in terms of EC10, EC50 of EEQ/AEQ.

XenoScreen XL YAS includes lyticase for a high sensitivity (10^-14M). XL stands for Extra Lyticase, which is responsible for higher signals of the β-galactosidase activity leading to higher sensitivity.

Characteristics

Includes all media, controls, also quality controlled plastic ware
Number of samples: 4 samples in the androgen receptor in duplicates, 8 dilutions or for 32 samples in 1 dilution.
Excel calculation sheet is available free of charge to support result Interpretation
Strains are not included in the kit and are available separately (20 passages).

Also available

YES (Yeast Estrogen Screen) Strain for max. 20 passages, art.no. N05-230-E (not included in the kit)
YAS (Yeast Andogen Screen) Strain for max. 20 passages, art.no. N05-230-A (not included in the kit)
YES YAS Culture Maintenance for growing the cells, art.no. N05-PMY-7
XenoScreen XL YES test kit for the determination of androgen agonistic activity, art.no. N32-233-Y
XenoScreen XL YES YAS test kit for the determination of estrogen and androgen agonistic plus antagonistic activity, art.no. N32-233-Y

XenoScreen XL YES Short Protocol

Short Protocol

XenoScreen XL YES Short Protocol

XenoScreen Leaflet

Leaflets

XenoScreen Leaflet

XenoScreen XL YES

MSDS

XenoScreen XL YES