Estrogen and Androgen Receptor Transscriptional Activation Assay Screening Test with Yeast Cells

Art. No.: N04-233-Y

4 Samples in 4 Receptors (estrogen, androgen, agonistic and antagonistic activity), duplicates, 8 dilutions. 8 Samples can be measured for agonistic activity only. 32 samples in 4 receptors, duplicates, if only 1 dilution is measured. YES and YAS strains (up to 20 passages) as well as YES YAS Culture Maintenance Set need to be added separately.

Product Description
Documents (3)

XenoScreen XL YES YAS is a ready to use estrogen and androgen receptor transactivation test kit for the identification of chemicals, agrochemicals, cosmetics (EDC's) or water samples with endocrine disruptive activity, i.e estrogenic and androgenic activity or antagonistic activity. In the receptor transactivation test a chemical is required to bind to the receptor and to activate the receptor. In compliance with OECD Guideline TG455 and ISO 19040. The kit can also be used for measurement of agonistic activity only allowing the measurement of 8 compounds.

Many endocrine disrupting chemicals adversely impact the estrogen or androgen signaling by interacting with estrogen or androgen receptors. The receptors show ligand selectivity and target gene regulation, they modulate metabolic enzymes that are critical for normal estrogen or androgen synthesis and metabolism. Typical EDC's are e.g. phytoestrogens, bisphenolics, and organochlorine pesticides. Many endocrine discrupting chemicals act through multiple mechanisms and therefore biological effects of EDCs need to be carefully interpreted.

In the receptor transcriptional activation assay, YES (Yeast Estrogen Screen) and YAS (Yeast Androgen Screen) cells are exposed to 8 concentrations of a test sample, as well as a positive and a negative control, for 18 hours (XenoScreen XL YES YAS). After exposure, the receptor mediated β-galactosidase expression is assessed via a chromogenic substrate. Each dose is tested in duplicate to allow for statistical analysis of the data.

A dose-dependent and significant increase or decrease in the color development relative to the corresponding negative control upon exposure indicates that the sample acts as an endocrine disruptor. Comparison with standardized positive controls (tested in multiple doses) allows for a quantification of the endocrine disrupting properties in terms of EC10, EC50 of EEQ/AEQ.

XenoScreen XL YES YAS includes lyticase for a higher sensitivity. XL stands for Extra Lyticase, which is responsible for higher signals of the β-galactosidase activity leading to higher sensitivity.

Characteristics for Estrogen and Androgen Receptor Transcriptional Activation Test Kit

Includes all media, controls, also quality controlled plastic ware
Agonistic and antagonistic activities: Estrogen-, Androgen Receptor agonistic and antagonistic activities,
Number of Tests: 4 samples in 4 different receptors if tested in duplicates, 8 dilutions and 32 samples in 4 different receptors if tested in 1 dilution. Allows for measurement of only 1 receptor activity whereby 4 times more samples can be measured.
Excel calculation sheet is available free of charge to support result Interpretation
Includes Lyticase to increase permeability
Strains are not included in the kit and are available separately, they can be cultured for 20 passages.

Other Products and Services for Estrogen and Androgen Receptor Assays

YES (Yeast Estrogen Screen) Strain for max. 20 passages, art.no. N05-230-E (not included in the kit)
YAS (Yeast Andogen Screen) Strain for max. 20 passages, art.no. N05-230-A (not included in the kit)
YES YAS Culture Maintenance for growing the cells, art.no. N05-PMY-7
XenoScreen XL YES test kit for the determination of estrogen agonistic activity, art.no. N32-233-Y
XenoScreen XL YAS test kit for the determination of androgen agonistic activity, art.no. N32-234-Y

XenoScreen Yes Yas XL Short Protocol

Short Protocol

XenoScreen Yes Yas XL Short Protocol

XenoScreen XL YES YAS

MSDS

XenoScreen XL YES YAS

XenoScreen Leaflet

Leaflets

XenoScreen Leaflet