250 µl after Reconstitution
can be aliquoted into 10 x 25 µL for 10 overnight cultures
in 10 mL growth medium for Ames Test in agar plates
TA1535[pSK1002] tester strain has been used for many years to detect potentially genotoxic test compounds in a 96-well microplate. Upon exposure of the bacterial cells to potentially genotoxic chemicals, biocides, environmental samples, surface, waste or drinking water, the umuC gene is induced as part of the general SOS response. The plasmid pSK1002 contains the umuC gene fused to the lacZ-gene which includes β-galactosidase. Once the umuC-gene is induced it can be assessed by determination of the β-galactosidase activity: conversion of the colorless ONPG substrate (o-nitrophenyl-β-D-galactopyranoside) to the yellow product o-nitrophenyl. As with other bacterial genotoxicity and mutagenicity assays, compounds requiring metabolic activation for activity can be investigated with the addition of metabolically induced S9 microsomal rat liver fraction.
Characteristics
Safety
In most countries the Ames S.typhimurium tester strains are categorized under the biosafety level 1 (Risk Group 1, Biological agents that are unlikely to cause human disease). Please contact your local authorities to verify biosafety level in your own country.
The host cell Salmonella typhimurium used in the UmuC test system contains genes from E.coli and is considered GMO. TA strains carrying the rfa mutation have a significantly reduced lipopolysaccharide layer and are not pathogenic. The strains do not disturb the biological diversity.
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