Ames MPF Penta 1 - 1 Sample Ames Test Kit with 5 Strains

Art. No.: C01-512-S2-P

5 x 48 Measuring Points: 1 Sample per Strain if tested in Triplicates, 6 Dilutions, +/- PB/ß-Naphtoflavone-induced S9, Negative and Positive Controls

Product Description
Documents (18)

Ames MPF Kit is a liquid microplate fluctuation assay kit, standardized and quality controlled. It is a ready to use Kit for the Ames mutagenicity assay and includes 10 vials each of semisolid TA98, TA100, TA1535, TA1537, E.coli WP2 uvrA, E.coli WP2 pKM101, Booster, Growth -, Exposure -, Reversion Indicator Media, Positive Controls, Post-Mitochondrial Supernatant S9 from lyophilized Phenobarbital/Naphtoflavone induced rat liver. The assay is using the same strains, positives controls and rat liver S9, cofactors as listed in OECD 471 and is quality controlled according to OECD 471. The liquid media used in this assay allow to test successfully pharmaceuticals, (agro-)chemicals, cosmetics, water, soil, many publication are referenced on this homepage. Especially volatile compounds, or genotoxic impurities available in low quantities, e.g. nitrosamines can be tested successfully with the Ames MPF (ICH M7).

Characteristics

Quality controlled kit including all strains, S9, media, positive controls - no inhouse quality control, no sterility control necessary
Certificate of Analysis is provided
Excel Calculation Sheet to support data Interpretation free of charge
Compliant with ICH M7 allowing testing for very small quantities of genotoxic impurities, e.g. nitrosamines
Suitable for volatile test compounds
Number of tests corresponds to 5 x 480 agar plates.
Standardized procedure
Shipped at room temperature from external storage in Germany or from Switzerland
High sensitivity and excellent correlation with agar plate test (see referenced publications)
4 times less test compound as compared to the agar plate test, optimized for screening purposes
Less hands-on-time and simultaneous processing of several replicates
Automation available - high throughput
3Rs - 12 fold less consumption of rat liver liver S9, significant less test animals sacrificed
Less error prone due to easy colorimetric read-out
Waste - Significant less plastic waste and thus reduced contaminated waste in Environment
Technology transfer available
Enough material to process strains individually

The Ames test is a bacterial mutation reversion assay which evaluates the mutagenic potential of chemical, pharmaceutical or cosmetic substances, food additives, food packaging material, mixtures, drinking-, waste water or soils. The bacterial strains carry several mutations that inactivate the gene involved in the synthesis of histidine (s.typhimurium) or tryptophan (E.coli), i.e. they grow only in a medium supplemented with either his or trp. Mutagens induce mutations which may restore the ability of the bacteria to re-synthesize the amino acid (his or trp) and to grow in medium without his or trp. Growth of bacteria induces a pH shift which is easily detected by the yellow collor due to the pH-indicator included in the medium.

Also available:

B01-513-S2-P Ames MPF Penta 2 with S9 from lyophilized PB/ßNaphtoflavone induced rat liver
C10-512 Ames MPF Penta 1, basic kit without S9 and positive controls
Individual products for Ames Test: S9, media, positive controls, etc.
D05-210 MicroAmes24, miniaturized Ames Test on 24 well agar plates
S9 Cofactor Kit (Art.No. PCO-0800)
Service Analytics for Ames Tests with all techniques under GLP and non GLP conditions (OECD 471), also for testing very low sample quantities in "µg" or for mixtures with NanoAmesTM, ultra-miniaturized Ames Test (ICH M7, 35 µg/compound in 5 strains)