Ames II

Art. No.: E10-213

10 Sample Kit when tested in 2 strains (TA98, TAMix) ,+/- S9, 3 Replicates, 6 Doses, Positive & Negative Controls: Strains, Media, Ampicillin. Not included in the kit: Rat liver S9, Controls. Not included in the kit: S9 cofactor solutions, microplates

Product Description
Documents (8)

Ames II is a miniaturized, ready to use Ames Test kit in a liquid microplate format. It includes all reagents necessary to run 10 samples with strains TA98 and TAMix (Mix of TA7001, TA7002, TA7003, TA7004, TA7005, TA7006): 10 vials each of frozen TA98, TAMix, plus Growth -, Exposure -, Reversion Indicator Media, Ampicillin. The kit does not contain Positive Controls, lyophilized Post-Mitochondrial Supernatant S9. Not included in the kit are plastic ware and S9 Cofactor solutions.
Published data about several tested compounds with this liquid microplate Ames Test are available under Database or Publications on this homepage.
The kits are quality controlled and do not need an inhouse quality control (phenotypes) like the traditional agar plate assay.

Service analytics for early drug discovery with test compound of 11 mg / strain (+/- S9) with the Ames II or Ames MPF assay system is available as well under GLP and non GLP conditions, please contact info@xenometrix.ch.

Characteristics

Quality controlled products: all products of the kit
Certificate of Analysis: Provided
Excel Calculation Sheet to support Data Interpretation
Comparison with agar late test: Corresponds to 2 x 480 agar plates.
Enough Material to process Strains individually
Shipped on dry ice from external Storage in Germany
High sensitivity and excellent correlation with agar plate test (see database and publications on this homepage)
4 times less test compound as compared to the agar plate test
Less hands-on-time and simultaneous processing of several replicates
Automation available - High throughput format
3Rs - 12 fold less consumption of rat liver liver S9, less test animals
Less error prone due to easy colorimetric read-out
Waste - Significant less plastic waste and thus reduced contaminated waste in environment

Also available

S9 Cofactor Kit (Art.No. PCO-0800) for preparation of S9 Mix
A10-210-S1-P Ames MPF 98/100 with S9 induced by Aroclor 1254 - shipped at RT
A10-210-S2-P Ames 98/100 with S9 induced by PB/ß-Naphtoflavone induced rat liver S9* - shipped at RT
Ames MPF 98/100 Aqua for environmental water samples - shipped at RT
Ready to use 5 Strain kits Ames MPF Penta 1 and Penta 2 - shipped at RT
Individual Reagents, such as rat liver S9

*strongly recommended over Aroclor induced S9, Aroclor being a strong environmental polutant and on the EC list of banned chemicals.