1

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P Gee, DM Maron, BN Ames; Detection and classification of mutagens: A set of base-specific Salmonella tester strains; Proc Nat Acad Sci USA (1994) 91, 11606-11610.

 

 

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2

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K Braun; Automation of the Ames II Assay: High Through-put screening of mutagenic substances; Aventis Pharma Deutschland GmbH, DI & A, Lead Optimization, Drug Safety Evaluation MipTec ICAR (2001).

 

3

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M Crook; Automation of the Ames II toxicology test; MipTec-ICAR (2000), P07.

 

4

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P Gee, CH Sommers, AS Melick, XM Gidrol, MD Todd, RB Burris, ME Nelson, RC Klemm, E Zeiger; Comparison of responses of base-specific Salmonella tester strains with the traditional strains for identifying mutagens: The results of a validation study; Mutation Res (1998) 412, 115-130.

 

 

 

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5

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WW Piegorsch, SJ Simmons, BH Margolin, E Zeiger, XM Gidrol, P Gee; Statistical modeling and analyses of a base-specific Salmonella mutagenicity assay; Mutation Res (2000) 467, 11-19.

 

 

 

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6

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G Engelhardt, E Jacob, R Jäckh; Ames II assay: Results of a validation study

 

 

 

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7

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V Gervais, D Bijot, N Claude; Assessment of a screening experience with the Ames II test and future prospects; European Environmental Mutagen Society Annual Meeting: From Hazard to risk, Aberdeen Scotland (UK) (2003) P120.

 

8

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S Flückiger-Isler, M Baumeister, K Braun, V Gervais, N Hasler-Nguyen, R Reimann, J van Gompel, H-G Wunderlich, G Engelhardt; Assessment of the performance of the Ames II assay: A collaborative study with 19 coded compounds; Mutation Res (2004) 558, 181-197.

 

 

 

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9

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S. Flückiger-Isler, Markus Kamber; The Ames MPF® 98/100 Assay: Novel Mutagenicity Testing in Liquid Microplate Format using S.typhimurium TA98 and TA100 (shown at the EEMS 2006 in Prague, CZ)

 

 

Click here to see the poster

 

10 

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S. Flückiger-Isler, Markus Kamber; The Ames MPF® Assays: Novel Mutagenicity Testing in Liquid Microplate Format using S.typhimurium TA98, TA100, TA1535 and TA1537 (shown at the SOT 2007 in Charlotte, USA)

 

 

 

Click here to see the poster

 

11 

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G. Streck, S. Flückiger-Isler, W. Brack; Comparison of pre-assay clean-up procedures for unveiling the mutagenic potency of sediment extracts (shown at the SETAG 2006 in Montreal, Canada)

 

 

 

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12

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M. Kamber; Comparison of 4 Cytotoxicity Assays Performed Sequentially on the Same Cell Sample (shown at the EEMS 2007 in Basel, CH)

 

 

 

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13

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W. Schoonen et al.; In vitro toxicity screening as pre-selection tool; European Pharmaceutical Review, Issue 3 (2008), 40-44

 

14

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U. Lübcke-von Varel, I. Löffler, W. Brack; Effect-directed analysis of mutagenic polyaromatic compounds in sediment extracts (shown at the 2. Internation Symposium Genotoxicity in aquatic systems: Causes, effects and regulatory needs 2008 in Dessau-Rosslau, Germany)

 

 

 

Click here to see the poster

 

15

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M. Kamber, S. Flückiger-Isler, G. Engelhardt, R. Jaeckh, E. Zeiger; Comparison of the Ames II and traditional Ames test responses with respect to mutagenicity, strain specificities, need for metabolism and correlation with rodent carcinogenicity; Mutagenesis (2009) 24, 359-366

 

 

 

Click here to download the publication
Click here for suplemental data 1
Click here for suplemental data 2

 

16

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S. Flückiger-Isler, M. Kamber; THE AMES MPF™ PENTA I ASSAY: Mutagenicity Testing in Liquid Microplate Format Using OECD Guideline 471 Compliant Strains S. typhimurium TA98, TA100, TA1535, TA1537 and E.coli WP2 uvrA plus E.coli WP2 [pKM101]; Poster shown at the ICEM 2009 in Florence 

 

 

 

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17

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Newsletter Issue 4 / October 2009

On the limits of miniaturization of the Ames test

Limited availability of test compounds necessitates to miniaturize early pre-clinical toxicity or mutagenicity assays such as the Ames test. We discuss the factors that may be limiting for miniaturized assays such as the liquid microplate versions Ames MPF/Ames II. Upon reducing the number of bacteria a point will be reached where the solvent controls approach zero and thus would no longer allow to calculate a reliable "fold induction". It is concluded that the Ames II / Ames MPF liquid microplate versions of the Ames test achieve an optimal level of miniaturization without compromising reproducibility and sensitivity, particularly towards weak mutagens.

 

 

 

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18

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Newsletter Issue 4 / October 2009

Weak mutagens in the Ames II / Ames MPF assay

Reducing the number of bacteria and amount of test compound in an assay like the Ames MPF should not compromise the sensitivity of the assay, and weak mutagens should still be detectable.

We have compared three typical "weak" mutagens in the Ames plate incorporation assay (from the NTP database) and in the Ames liquid microplate format. The detailed results can be seen on our homepage

 

 

 

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19

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Newsletter Issue 4 / October 2009

Historic solvent control values (DMSO) for the PENTA I strains

 

 

 

Click here to see the data

 

20

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S. Flückiger-Isler, M. Kamber: Mutagenic responses in the Xenometrix Ames MPF PENTA I assay compared to published Ames plate incorporation data

 

 

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21

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C.V. Chandrasekaran, P. Thiyagarajan, K. Sundarajan, Krishna S. Goudar, M. Deepak, B. Murali, J. Joshua Allan, Amit Agarwal; Evaluation of the genotoxic potential and acute oral toxicity of standardized extract of Andrographis paniculata (KalmCold^TM); Food and Chemical Toxicology (2009) 47, 1892-1902

 

 

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22

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C.V. Chandrasekaran, K. Sundarajan, Kripalini David, Amit Agarwal; In vitro efficacy and safety of poly-herbal formulations; Toxicology in Vitro (2010) 24, 885-897

 

 

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23

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G. de Aragão Umbuzeiro, C.M. Rech, S. Correia, A.M. Bergamasco, G.H. Lima Cardenette, S. Flückiger-Isler, M. Kamber; Comparison of the Salmonella/Microsome Microsuspension Assay with the new Microplate Fluctuation Protocol for Testing the Mutagenicity of Environmental Samples; Environmental and Molecular Mutagenesis (2010) 51, 31-38

 

 

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