Estrogen- Androgen Receptor Transcription Activation Assays

Yeast Estrogen-, Androgen Receptor Screening Tests for chemicals or environmental samples, Agonistic and Antagonistic activities,

XenoScreen YES YAS and XenoScreen XL YES YAS  are rapid, high-sensitivity, combined endocrine disruptor bioassay with YES (yeast estrogen screen) and YAS (yeast androgen screen) for the determination of hormonally active compounds (endocrine disruptors) in environmental, chemical, agrochemical, biocide, pesticide and cosmetic samples.

XenoScreen YES YAS and XenoScreen XL YES YAS  are in vitro estrogen and androgen receptor transcriptional activation assays, each kit measures 4 receptor activities. The testkits are designed to identify both activating (agonistic) and inhibiting (antagonistic) properties of test samples but allow also to test for agonistic activity only. Fresh water, waste water, aqueous extracts and leachates, or chemical, agrochemical or cosmetic compounds, biocides, pesticides, plasticizers, detergents, fire retardants, UV filters or mixtures can be tested for endocrine disruption. The bioassay XenoScreen XL YES YAS includes additional lyticase for increased sensitivity.

Your benefits:

  • High sensitivity up to: 4 x 10-12 M (LoD; XenoScreen XL YES YAS)
  • Standardized test with certificate of analysis including step-by-step instruction manual for reproducible results
  • Simultaneous analysis of activating (agonist) and inhibiting (antagonist) behaviour
  • Results within 48 hours for XenoScreen YES YAS and 18 hours XenoScreen XL YES YAS
  • 1–2 hours hands-on time
  • 4 x 96 measuring points allowing different assay configurations: One suggested configuration for XenoScreen YES YAS (2 receptors, agonistic and antagonistic activities) allows analysis of 5 samples and for XenoScreen XL YES YAS (2 receptors) of 4 samples each in duplicate, 8 dilution steps, detection of agonist and antagonist activities, with positive and solvent controls. Other assay configurations are also possible.
  • Assay for concentrated or diluted samples requiring 73 μl or 16 μl (XL) as little as sample volumes
  • Excel calculation sheet for evaluation of results. Xenometrix also offers the evaluation of results to customers free of charge.

Test Kits

Yeast Estrogen Screening Test Kit - Agonistic Activity

Bioassay “XenoScreen XL YES” and “XenoScreen XL YAS” have both stably transfected human estrogen receptors and measure qualitatively or quantitatively compounds with agonistic hormonal activity on the estrogen receptor or androgen receptor only. The kits include all media, controls, plastic material (except pipette tips) for testing 32 environmental samples, e.g. drinking, surface or waste water, in one concentration. A detailed instruction manual as well as an excel calculation sheet for data evaluation are provided.

Test Kit

Yeast Androgen Screening Test Kit - Agonistic Activity

Bioassay “XenoScreen XL YES” and “XenoScreen XL YAS” have both stably transfected human estrogen receptors and measure qualitatively or quantitatively compounds with agonistic hormonal activity on the estrogen receptor or androgen receptor only. The kits include all media, controls, plastic material (except pipette tips) for testing 32 environmental samples, e.g. drinking, surface or waste water, in one concentration. A detailed instruction manual as well as an excel calculation sheet for data evaluation are provided.

Test Kit

Yeast Strains

The common Baker’s or Brewer’s yeast (Saccharomyces cerevisiae) was genetically modified to identify compounds that can interact with the human estrogen and androgen receptors hERα and hAR. For this purpose the DNA sequences of hERα or hAR are stably integrated into the main chromosome of yeast cells. Additionally, the cells also contain an expression plasmid carrying the reporter gene lacZ encoding the enzyme β-galactosidase and estrogen (YES) or androgen (YAS) responsive elements.

Yeast cells can be stored frozen at -20°C for up to 4 months or at -80°C or in liquid nitrogen for several years.

The Strains

Yes, Yas Culture Maintenance Set

The YES YAS Culture Maintenance Set is used to store and regrow Yeast Strains. Cell cultures with Xenometrix Yeast Strains can be stored for up to 2 weeks in growth medium (included in YES YAS Culture Maintenance Set) in the refrigerator. The overnight culture is started by diluting stored culture with growth medium and incubation at 31°C.

Test Kit